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Police want to hear from anyone who saw a crash that left the Air Balloon roundabout closed for over six hours.
The collision was between a black Rover 620 saloon and a red Mercedes curtain sided truck and happened at around 1.40pm on Monday.
The driver of the Rover, a 60-year-old woman from Nailsworth, was taken by ambulance to hospital but died a short while later.
The passenger in the Rover, a 35-year-old woman from Stroud, was treated at hospital for minor injuries.
The driver of the lorry, a 34-year-old man from Bicester, suffered minor injuries which did not require hospital treatment.
Anyone with any information about the collision is asked to contact Gloucestershire Constabulary on 0845 090 1234 quoting incident number 241 of November 8.