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The A5130 at Woburn Sands level crossing is closed from 12.01am on Saturday 20th March until 5am on Monday 22nd March.
It's so Network Rail can do some technical inspection work like checking under the sleepers, rails and ballast.
The road will re-open earlier if the work's finished in time.
There's a diversion in place which will be sign posted and is as follows:
Via Hardwick Road, The Leys, Bow Brickhill Road Woburn Sands, and continuing into Woburn Sands Road Bow Brickhill, Station Road Bow Brickhill and then right onto V10 Brickhill Street to the Walton Park Roundabout. Turn right and continue on A4146/H10 Bletcham Way to the Brown’s Wood Roundabout, turn left onto A4146/V11 Tongwell Street and continue in a northerly direction to the Walnut Tree Roundabout and turn right onto H9 Groveway to the Kingston Roundabout and to return to A5130 Newport Road on the fourth exit and vice versa.