Roar Katy Perry Download 'Roar' on iTunes
Over the last eight weeks the sailing village in Portland has played host to more than 650 sailors and officials.
Weymouth and Portland Borough Council said:
"In order to accommodate security fencing around parts of the athlete village a one way system was put in place on two roads in Portland. The fencing was an essential part of plans to ensure the highest level of security and safety during the Games.
As the London 2012 Olympic and Paralympic Games have come to an end, the security fencing will be removed and the village made ready to hand back."
To remove the fencing safely, Victory Road will be closed to all traffic between 21.30hrs on 12 September to 07.30hrs on the 13 September 2012, and Castle Road closed between 21.30hrs on 13 September to 07.30hrs on the 14 September 2012.
A signed diversion route will be operating during this time along Lerret Road, Mulberry Avenue and Liberty Road.
For the duration of the closure, access to the hospital for emergency vehicles will be maintained.